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Description / Abstract
The human papillomavirus (HPV) contains hundreds of serotypes that have been divided into low-risk versus high-risk variants. Whereas low-risk serotypes have been shown to cause benign skin lesions, the high risk serotypes are associated with various types of cancer, including cervical cancer. Research has demonstrated that high-risk HPV encodes an oncoprotein E7 that binds to and degrades the tumor suppressor retinoblastoma protein pRb. This project investigated the use of retroviral infection to establish a stable cell line to be characterized via western blot protein analysis. Both BJ and HT-1080 cell lines infected with HPV retroviral particles showed significant decreases in pRb expression in the western blot assay. In addition, the hydrophobicity of E7 protein sequences of low risk and high risk HPV serotypes was analyzed using the Grand Average of Hydropathy (GRAVY) calculator. Analysis of 24 well-classified serotypes demonstrated a negative correlation between risk level and hydrophobicity. Future experiments are needed to verify the presence of the viral protein E7 in HPV infected cells in order to confirm the linking of pRb degradation to E7 function.
Arete: The PLNU Honors Journal
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KeywordsCancer, Virus, HPV, Hydrophobicity, E7, Arete: The PLNU Honors Journal
Degree AwardedB.S. in Biology
Awarding InstitutionPoint Loma Nazarene University
Committee MembersHeidi Woelbern, Ariane Jansma, Mike Dorrell
Supervisor or ChairHeidi Woelbern
Peer Reviewed?No
Collections With This Resource
PLNU Undergraduate Honors Projects 2021-2025
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